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Part:BBa_K515107:Experience

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-12)


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SCAU-China

The main goal of this characterization is to find out which pH value is the most effective among the 6 pH values in order to obtain a suitable culture condition for the expression of Dendar2.We further found that the part which expressed the protein Dendar2 worked well in E.coli strain DH10B, like in the DH5 alpha.

Result and Findings

1.pH 4.5 is found to be the best pH that is suitable for the expression of Dendar2 .

2.Thered fluorescence from Dendar2 after photoconversion showed stronger fluorescent intensity than before.

3.Compared with the the expression pattern of Dendar2 as showed previously in DH5α, we concluded that the expression of Dendar2 works in both E.coli strains.

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